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Antibody Target: Beclin 1

Biovision公司Beclin 1 Antibody

Alternative Name: BECN1 , GT197 , Beclin-1 , beclin1 , ATG6 , VPS30

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Immunogen: Synthetic peptide corresponding to residues around amino acid 25 of mouse Beclin 1

Accession#: O88597

Gene ID: 56208

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg （0.2mg/ml） protein A purified rabbit anti-Beclin 1 polyclonal antibody in phosphate-buffered saline （PBS） containing 1% BSA, 50% glycerol, and 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse

Application: Western blot

Application & Usage: Western blotting （0.5-2 µg/ml）. However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~53 kDa beclin 1 in Jurkat cell lysate. A weak band of ~110 kDa （possibly dimer） can also be observed in Jurkat cells. In mouse intestine tissue lysate, the antibody recognizes a ~100 kDa band （possibly dimer）. Reactivity to other species has not been tested.

Biovision公司Beclin 1 Antibody

Storage Temp.: -20 °C

Shipping: gel pack

Background Descriptions: Beclin is a Bcl-2 binding protein. Its structure includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal （NES）. Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since over expression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects.

Handling: The antibody solution should be gently mixed before use.

Usage: For Research Use Only! Not to be used in humans.

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